Human TaqI RFLP recognized by neurofilament gene probes

A TaqI RFLP for the human arylsulfatase A gene.

TaqI RFLP at D21S137.

Chromosomal Localization: The 3 polymorphic bands were mapped to chromosome 22 using a somatic cell hybrid panel. The 2.1 kb constant band mapped to chromosome lq whereas all other constant bands mapped to chromosome 20. Whereas the active gene for hTOPl is located on chromosome 20ql 1.2—13.1, two pseudogenes are on chromosomes Iq23—24 and 22qll.2-13.1 (2). The sequences of hTOPl and the two ps...

TaqI RFLP polymorphism of the ovine complement component C4 gene.

Protocol: Reactions were performed as described (2) using 1 ng of DNA and 100 pmoles of each primer. DNA was amplified for 20 cycles using a Perkin Elmer Cetus DNA Thermal Cycler: denaturation, 94°C, 1 min; annealing, 60°C, 1 min; and extension, 70°C, 1.5 min. Ten /il of the reaction was analyzed directly on a 2.5 % NuSieve GTG agarose gel run in Tns-borate buffer. The amplified DNA fragments v...

A Taqi RFLP of the human TGF alpha gene is significantly associated with cutaneous malignant melanoma.

A TaqI restriction fragment length polymorphism (RFLP) of the human transforming growth factor alpha (hTGF alpha) locus was analyzed in DNA from 63 normal individuals, 34 malignant melanoma (MM) cell lines, and 18 melanoma biopsy specimens. The frequency of a 2.7-kb allele (0.18) in MM cell lines was significantly higher (p less than 0.01) than in lymphoblastoid cell lines (LCLs) derived from u...

Bgl II RFLP recognized by a human IRBP cDNA localized to chromosome 10.

SOURCE/DESCRIPTION: A 2184 bp cDNA (H.4 IRBP) encoding human interstitial retinol-binding protein (IRBP; Fong et al., 1984) isolated from a human retina cDNA library in XgtlO by screening with a bovine IRBP cDNA probe (B.23; Liou et al., 1986). POLYMORPHISM: Bgl II (AGATCT) identifies a 2-allele polymorphism with bands at 6.3 kb (A1) and 4.3 kb (A2). There is a strong invariant band at 2.5 kb. ...